Atcc hl-60

4825

The human promyelocytic leukemia cell line HL-60 (ATCC® CCL240™) was purchased from the American Type Culture Collection (ATCC, USA). HL-60 cells were commonly cultured in RPMI 1640 medium supple-mented with 10% (v/v) fetal bovine serum containing 100 U/ml penicillin and 100 U/ml streptomycin at 37 °C in a humidified atmosphere with 5% CO 2

Cell Line Description: The HL-60 cell line is a human acute myeloid leukemia cell line. The HL-60 cell line is included in the genetic alteration cell panel MYC (ATCC® TCP-1035 ™). Spin down before use. Verified mutation in parental cell line: MYC amplification.

Atcc hl-60

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null, p.M1_* 394del  center. We optimized the NCI60 cell line panel for generating 3D spheroids of a prespecified diameter (300–500 µm) in ultra-low 20 A549/ATCC. 7500 33 HL -60. 40,000. Leukemia.

HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e.

Atcc hl-60

ATCC Genome Portal. The ATCC Genome Portal makes it easy to find the high-quality whole-genome sequencing data needed for your research.

The prostate cancer cell lines LNCaP (ATCC, Manassas, VA, USA; ATCC CRL-1740) and PC3 (ATCC CRL-1435) as well as the myeloid cell lines THP-1 (ATCC TIB-202) and HL-60 (ATCC CCL-240) wereusedinthisstudy.

Atcc hl-60

Verified mutation in parental cell line: MYC amplification. Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service … hl-60-luc2 atcc ® ccl-240-luc2 ™ frozen 1.0 mL For-Profit: $1,310.00 Non-Profit: $825.30 hl-60/s4 atcc ® crl-3306 ™ frozen 1.0 mL For-Profit: $632.00 Non-Profit: $537.20 HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e. HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e.

No PMID.

Atcc hl-60

A Hematoxylen-Eosin stained paraffin section of a subcutaneous HL-60 xenograft is shown on the right. As routine quality controls, the cells are regularly checked for Mycoplasma contamination and authenticity (via STR DNA Typing). Expression data using western … Article Snippet: Cell lines The mycoplasma-free AML U937 (ATCC CRL-1593.2; French-American-British/FAB phenotype M5, monoblast), NB4 (M3, promyelocyte) [ ], HL-60 (ATCC 240-CCL; M2, myeloblast) and THP-1 (ATCC TIB-202; M5, monoblast) cells were cultured in complete RPMI 1640 medium supplemented with 5% FCS in a 5% CO2 humidified atmosphere at Human Aml Cell Lines Hl60, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 2 PubMed citations. Cell culture Human acute promyelocyte leukemia HL-60 cells and acute myeloid leukemia KG1a cells were purchased from the American Type Culture Collection (ATCC)(Manassas, VA, USA) and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) (Gibco, USA), penicillin (100 U/mL, Gibco), and streptomycin (100 µg/mL, Gibco). ..

ref. consensus. comment HL-60, CCL-240, R248L, 1018. p53 null. null, p.M1_* 394del  center. We optimized the NCI60 cell line panel for generating 3D spheroids of a prespecified diameter (300–500 µm) in ultra-low 20 A549/ATCC.

Atcc hl-60

This reference provides a recommended procedure to transfect plasmid DNA into HL-60 Human lymphoblasts (ATCC Cat. No. CCL-240) using Lipofectamine® LTX Reagent (Cat. No. 15338-100). 7klvsurgxfwlvlqwhqghgiruoderudwru\uhvhdufksxusrvhvrqo\ ,wlvqrwlqwhqghgiruxvhlqkxpdqv :kloh$7&&xvhvuhdvrqdeohhiiruwvwrlqfoxghdffxudwhdqgxs wr Green columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with N , N -dimethylformamide (DMF). Gray columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with DMF and activated with phorbol 12-myristate 13-acetate (PMA).

It is notable that  Some ATCC cell, are shipped as growing cultures in culture vessels. DMSO can cause some cells to differentiate (e.g., HL-60 promyeloblast cells) and may be  An evaluation of the effects of the compound on the cell cycle of HL-60 cells and and HL-60 (promyelocytic leukemia, ATCC CCL-240), donated by Professor  While HL-60 and HL-60/S4 are recognized as different cell lines by ATCC, they are both used for similar studies [22,24]. It is notable that despite the HL-60/S4  No cell line information in ATCC. DSMZ.

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Hl60 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36–48 hours. ATCC Genome Portal. The ATCC Genome Portal makes it easy to find the high-quality whole-genome sequencing data needed for your research.